Human plasma derived drugs separation by fractionation of plasma with polyethylene glycol

human plasma derived drugs separation by fractionation of plasma with polyethylene glycol

background: there are varieties of purification techniques for separation of human plasma proteins such as salting out, ion exchange chromatography, and ethanol fractionation. there are limitations for each method, for example in salting out method, the salt has to be removed in an additional step. ion exchange chromatography is difficult for scaling up, and plasma fractionation is a time consu...

Human Plasma Derived Drugs Separation by Fractionation of Plasma with Polyethylene Glycol

Human blood as a unique tissue (1), contains many important proteins with therapeutic uses (2). As a biological drugs, virus inactivation is one of the crucial steps in their preparations of these plasma derived proteins (3-6). The most important proteins in human plasma are: albumin, immunoglobulin, fibrinogen, and coagulation factors VII, VIII and IX (7). There are varieties of purification t...

Enhanced Detection of Low-Abundance Human Plasma Proteins by Integrating Polyethylene Glycol Fractionation and Immunoaffinity Depletion

The enormous depth complexity of the human plasma proteome poses a significant challenge for current mass spectrometry-based proteomic technologies in terms of detecting low-level proteins in plasma, which is essential for successful biomarker discovery efforts. Typically, a single-step analytical approach cannot reduce this intrinsic complexity. Current simplex immunodepletion techniques offer...

Plasma expansion by polyethylene-glycol-modified albumin.

Systemic inflammatory response conditions are associated with capillary leak and haemodynamic compromise. Fluid resuscitation to reverse the ensuing hypovolaemia is, however, complicated by the decreased endothelium reflection coefficient to albumin and other colloids. We developed PEG-Alb (albumin covalently linked to polyethylene glycol) as a potential resuscitative agent. PEG was covalently ...

Plasma Fractionation in Malaysia

Immunoglobulin A Preparation from Human Pooled Plasma Using Plasma Fractionation and Ion Exchange Chromatography

Background: In this study we have prepared the IgA solution from normal human plasma using plasma fractionation and ion exchange chromatography. Materials and Methods: Using fractionation of plasma with cold ethanol (starting with 8% ethanol), fraction III was prepared as a suitable source for IgA preparation. Then it was treated with caprylic acid for separation of impurities. For enrichm...